51116 DATASHEET PDF
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Antibodies are purified by protein A and peptide affinity chromatography. Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins 3.
Prepare solutions with reverse osmosis deionized RODI or equivalent grade water. Would you like to visit your country specific website?
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FXR2 Antibody – Application Dilutions Western Blotting 1: To Purchase S View sizes. Do not aliquot the antibody. Blotting Membrane and Paper: Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity.
Wash three times for 5 min each with 15 ml of TBST. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human FXR2 protein. Find answers on our FAQs page. Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation 1.
Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so datashee signal attributed to binding of the new datashert is not leftover signal from the first immunoblotting experiment.
Primary Antibody Dilution Buffer: It should be noted that for the best possible results a fresh blot is always recommended. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water.
(PDF) TPS51116 Datasheet download
This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. Aspirate media from cultures; wash cells with 1X PBS; aspirate.
Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal.
Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Detection of Proteins Directions for Use: Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
Changing to another country might result in loss of shopping cart. Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.
These results suggest that fragile X syndrome is related to abnormal translation caused datasheet defects in RNAi-related pathways.
Microcentrifuge for 5 min. Treat cells by adding fresh media containing regulator for desired time. Proceed with detection Section D. Dilute to 1X with datassheet 2 O. From sample preparation to detection, the reagents you need for your Western Blot are datsheet in one convenient kit: Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.
TPS from Texas Instruments
Protein Blotting A general protocol for sample preparation. More about how we get our images. Electrotransfer to nitrocellulose membrane Incubate substrate with membrane for 1 minute, remove datasueet solution membrane remains wetwrap in plastic and expose to X-ray film. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Biotinylated Protein Ladder Detection Pack: