TRAITS. Goniopsis cruentata, commonly known as the mangrove root crab, is about cm in length at maturity, with a square dark brown carapace that varies in. The present study reports the use of biomarkers analyzes in mangrove root crab Goniopsis cruentata tissues to assess the environmental quality of two tropical. Inhabits brackish muddy waters, often in mangrove swamps (Ref. ), able to move fast between the roots and trunks of trees (Ref. ). Found in burrows .
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Log In Sign Up. Biomarkers in mangrove root crab Goniopsis cruentata for evaluating quality of tropical estuaries. S 13 DOI: MERE To appear in: Marine Environmental Research Received Date: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.
Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Biomarkers in mangrove root crab Goniopsis cruentata for evaluating quality 2 of tropical estuaries 3 4 5 PT 6 Authors in order: Super Quadra NorteBloco J, ap.
Cholinesterase, Glutathione-S-transferase, DNA Damage, coastal zone, 48 contamination, crabs, estuary, protected area. PT 54 – Effect on crabs show that legal protection status of CR estuary is not effective. Introduction 57 58 Estuarine systems are among the most threatened environments around the 59 world, and especially in tropical and subtropical areas, they are associated with urban 60 occupation including major cities, ports and industrial complexes.
Frequently, there are no specific regulations to their management due to the lack RI 64 of a defined legal status Dauvin and Ruellet,and the creation of protected areas 65 become the major strategy widespread throughout many countries to preserve or restore SC 66 coastal and estuarine sustainability Brito, Biomarkers include suborganismal endpoints, which TE 76 allow detection of stress at sublethal levels early-warning Depledge and Fossi, Although biomarkers evaluation was established about 25 years ago NRC, C 81they still have been poorly used to assess tropical environment quality.
At same 82 time, there is a global effort to identify good biomarkers for tropical environments and AC 83 set up monitor species, which occupy different ecological niches and represent different 84 functional groups Jha, Environmental Risk Assessment ERA requires conceptual models to 88 understand how stressors may affect natural environment.
Often, some taxonomic 92 groups have been used for monitoring and assessing effects of pollution, as mollusks 93 Bainy et al. Grapsidae to evaluate the environmental integrity of a tropical estuarine 99 area under anthropic pressure. This initial investigation employed well-established SC biomarkers commonly used in temperate species, as measures of DNA damage, cholinesterases ChE and glutathione-S-transferase GST activities, in order to U evaluate the feasability of G.
Material and methods M 2. Study area, sentinel organism and sampling The tropical estuarine systems chosen for this study represent two of the major D rivers of Fortaleza Metropolitan Region FMR. Contributions from diffuse sources related to irregular land occupation, urban drainage, storm water runoff, agriculture and small boat traffic also AC occur Cavalcante et al.
Moreover, CR sediments were previously considered contaminated by metals Marins et al. On the other hand, Pacoty River PR has about km length, and its estuarine zone is occupied by relatively well-conserved mangrove. Additionally, it represents an alternative food resource for traditional communities Souza and Silva, SC The PR and CR stations were established considering the U occurrence of well-established mangroves on their respective vicinities and the limits of protected areas e.
Three sampling stations were established in CR, at the same sites of those used by Nilin et al this M issuewho considered the natural estuarine gradients, the localization of pollution sources and influence of Maranguapinho River figure 1: CR1 was situated upstream Maranguapinho confluence, CR2 was placed in confluence area, and CR3 was D positioned downstream, closer to the estuary mouth.
Goniopsis cruentata individuals were manually captured at AC low tide. The sampling effort on CR stations consisted in one hour or a minimum of 10 animals for each station.
At PR, a total of 30 animals were captured. Immediately, organisms were transported alive to the laboratory in thermal isolating boxes to maintain temperature stabled avoiding high temperatures during the transportcontaining 2cm layer of water from the collection site. In laboratory, animals were processed for analysis within 12 h after sampling. Biomarkers The specimens of G.
Haemolymph was collected for comet assay by puncture between pereiopods segments with a hypodermic syringe 22G needle previously washed with EDTA 10 PT mM, Sigma-aldrich and containing 1: Within one week, the gills were gradually U thawed on ice, homogenized in ice cold potassium phosphate buffer 0.
All biochemical analyses were done within 3 weeks after the tissue homogenization. The gill tissues were used for biomarker analyses due to their functions: Biochemical analyses AC The gills were gradually thawed on ice, homogenized in potassium phosphate buffer 0. The dosage was done in quadruplicate.
RI The cholinesterase activity ChE was carried out using the method described by Ellman et al. M 2. Genotoxicity analysis The comet assay single cell gel assay was performed and analysed according to D Villela et al.
TE cruentata characteristics. The slides were EP submerged in lysis buffer NaCl 2. Afterwards, slides were submerged C in unwiding alkaline buffer min NaOH 0. After AC electrophoresis, the slides were submerged in neutralizer solution cruentataa 15min. In the dark, the slides were re-hydrated in distilled water 5min.
Two slides were PT prepared for each specimen, cells were analyzed under optical microscope 50 cells per slide. For damage index calculation, cells were classified into five classes based on the length of migration and on the amount of DNA in the tail figure 2. Statistical analysis AN The relationship between biomarker responses and weight of crabs according d by linear regression, considering data from each organism. Results and Discussion EP The present study analysed genotoxicity and enzymatic changes in tissues from C the land-crab G.
Abiotic variables measured in field indicated expected conditions for tropical estuarine environments: These values are in agreement with the standards established to Class 1 brackish water bodies by Brazilian federal legislation, since the studied estuaries are legally protected areas Brasil, The size classes of G.
Moreover, a wide variability on activity range in Pacoti River PR crabs could be observed, as shown by high standard deviations. Exposure to different compounds may lead to different GST responses in the tissues Bainy et al.
WoRMS – World Register of Marine Species – Goniopsis cruentata (Latreille, )
As in this investigation GST activity was goniopss in crabs from all CR stations, and that literature reports to the presence of metals and organic compounds in CR, we can assume that these responses were due to contamination in CR. Results obtained for ChE activity are RI presented in figures 5 and 6. Significant inhibition in ChE activity was observed in the crabs from CR1; whereas potential effect on CR2 gpniopsis was observed for both SC anterior and posterior gills; incipient effect appear to occur in PR crabs anterior gills.
Organophosphate pesticides and carbamates are among TE major inhibitors of ChE activity in vertebrates and invertebrates Fulton and Key, ; Guilhermino et al.
Thus, considering the proximity of a potential source of organophosphates agriculture area about m from CR1 and the observed effects for ChE, specifically for this class of contaminants, CR1 appears to present a worse condition, followed by CR2 and then by CR3.
Some authors link inhibition of ChE to alterations in physiology of decapods, e.
Moreover, Vieira et al. Thus, the inhibition pattern observed in this study could lead to an AN impairment of neurological functions of G. M Salinities at the moment of collection do not seem to have influenced the GST activity in G. In case of ChE, the observed results could not to be D related to salinity changes as well e.
Some studies evaluated the cruenyata of salinity alterations due to tidal cycle on estuarine organisms. Nevertheless, Cailleaud et al. A range of compounds present direct and indirect effects in DNA integrity of estuarine organisms: PT sapidus crab related to organic contamination in water; Villela et al.
DNA damage could lead to incomplete transcription, cellular SC impairment, growth inhibition and negative effects in reproduction, immunity and other deleterious effects in organisms Woo et al.
The present study shows that the U crabs from CR exhibit Cruentsta damages according to a fashion trends opposite to the boniopsis gradient contamination described by Nilin et al this issue.
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Therefore, high AN DNA damage at CR3 seems to highlight the existence of different form and source of pollution which require further attention and investigation. Such results seem to be connected, cruehtata long-term exposure to contamination may cause inhibition in antioxidant system and then toxicity effects due reactive oxygen species may occur Regoli et al. Cavalcante et RI al. Bioaccumulation studies did not found TE contamination by Cu and Hg in oysters from this site Lopes et al.
Nevertheless, Lacerda et al. C Literature concerns to contamination complexity in CR, in special the presence of Cu, as several sources for this element occurs cruetnata CR basin, thus some attention AC must be given to this contaminant.
ADW: Goniopsis cruentata: CLASSIFICATION
PT After a contaminant exposure, intoxication may occur by direct action of contaminants on targets or by sub-products formed along depuration process; ultimate effect for exposed organism may be enzyme deactivation, lipid peroxidation, DNA damage and RI cells death Almeida et al.
Moreover, some transition metals e. The observed results are supported by literature M information on contamination levels for both estuaries, which show that PR presents good conditions whereas CR is impacted by several pollution sources. Results suggest that biota from CR is threatened; thus this estuary, as a legally protected area, demands D management actions for conserving such region, preserving the uses and quality of TE natural resources, and assuring the conservation of biological diversity.
In the case of CR estuary, a management plan was EP prepared SEMACE, but was not officially published nor implemented until the present; in its turn, PR estuary has not a management plan even after 10 years since its C creation. Conclusions Mangrove root crabs from CR estuary seem to be affected by pollution, as animals from this area exhibited adverse responses for all employed biomarkers.
The PT contamination of CR seemed to be related to multiple contaminants and sources. PR estuary did not exhibit signs of environmental stress. Regarding to the test species, G.
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